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. 2012 Dec 14;5(3):929–934. doi: 10.3892/ol.2012.1077

Figure 3.

Figure 3

Effects of FOXO3a downregulation by siRNA transfection on FOXO3a expression and apoptosis in MDA-MB-453 cells. (A) MDA-MB-453 cells were transfected with 100 nM siRNA control or the siRNA duplexes against FOXO3a mRNA. Forty-eight hours after transfection, the cells were treated with 4.0 μM NOC for 24 h. Western blotting of p-FOXO3a, FOXO3a, caspase-3 and caspase-9 was completed to confirm the downregulation of FOXO3a and the effects of caspase-3 and -9 cleavage by siRNA transfection. β-actin was used as the loading control. (B) MDA-MB-453 cells were transfected with 100 nM siRNA control or the siRNA duplexes against FOXO3a mRNA. Forty-eight hours after transfection, cells were treated with 8.0 μM NOC for 24 h. The apoptotic rate was analyzed by FCM using PI staining. Data shown are means ± SD (n=3). *P<0.05; **P<0.01 vs. 0.1% DMSO; #P<0.05 vs. the same concentration of NOC in combination with siRNA FOXO3a transfection. FOXO3a, forkhead box O3a; NOC, 7-dihydroxy-8-nitrochrysin; FCM, flow cytometry; PI, propidium iodide; SD, standard deviation; DMSO, dimethyl sulfoxide.