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. 2012 Nov 26;12:90. doi: 10.1186/1472-6750-12-90

Table 1.

Reaction times and yields for the oligosaccharide synthesis by the fructosyltransferase Suc1 from A. niger

 
t
Conversion
  [min] [% mol mol-1]
1-kestose
18
81
1-nystose
60
93
MF2
60
71
MF3
180
87
GF2
420
44
GF3
960
65
XF2
20
75
XF3
120
94
FF2
60
65
FF3 120 88

Reaction conditions were Suc1 enzyme dilution 1:50 (v/v), 500 mM sucrose analogue to be converted in Sörensen`s phosphate buffer (50 mM, pH 5.6) at 45°C and 200 rpm. The reaction time depending on the oligosaccharide to be synthesized is indicated.