Table 1.
Reaction times and yields for the oligosaccharide synthesis by the fructosyltransferase Suc1 from A. niger
| |
t |
Conversion |
|---|---|---|
| [min] | [% mol mol-1] | |
| 1-kestose |
18 |
81 |
| 1-nystose |
60 |
93 |
| MF2 |
60 |
71 |
| MF3 |
180 |
87 |
| GF2 |
420 |
44 |
| GF3 |
960 |
65 |
| XF2 |
20 |
75 |
| XF3 |
120 |
94 |
| FF2 |
60 |
65 |
| FF3 | 120 | 88 |
Reaction conditions were Suc1 enzyme dilution 1:50 (v/v), 500 mM sucrose analogue to be converted in Sörensen`s phosphate buffer (50 mM, pH 5.6) at 45°C and 200 rpm. The reaction time depending on the oligosaccharide to be synthesized is indicated.