Figure 5.

A. Immunoblot of total cell lysate from 293 cells transfected with pEGFP vector or pEGFP-SON probed with antibodies against EGFP or SON.B. Fluorescence images of 293 cells transfected with pEGFP-SON or pEGFP-vector. Note that EGFP-SON was speckled in the nuclei of interphase cells and diffusely dispersed in mitotic cells. Original magnification, 600×. C. Time-lapse images of 293 cells transfected with EGFP-SON. Sequential images taken at 10-minute intervals are shown (the number on each image indicates minutes from starting). EGFP-labeled SON dispersed in the cytoplasm during metaphase and anaphase (panels at 0 and 10 minutes), accumulated in small foci in the cytoplasm during telophase and cytokinesis (panels between 20 and 50 minutes), and then gradually reassembled in nuclear speckles (panels at 60 minutes and later). Original magnification, 400×.