Figure 3. Morbillivirus V proteins show better ability to block IFNα-induced STAT1 and STAT2 phosphorylation than INFγ-induced STAT1 phosphorylation.

A549 cells were transfected with 1 µg of empty vector or plasmid driving the expression of the indicated protein. Twenty hours post-transfection, cells were mock treated or treated with (a),(b) 1000 IU/ml of IFNα or (c) 1000 IU/ml of IFNγ for 30 minutes, fixed with PFA followed by methanol and stained with monoclonal anti-V5 tag and (a), (c) anti-phospho-STAT1 (STAT1P) or (b) anti-STAT2 as in “Material and methods”.. Representative confocal images from three independent experiments are shown.