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. 2013 Feb 19;8(2):e55922. doi: 10.1371/journal.pone.0055922

Figure 5. Apigenin enhances Apo2L/TRAIL-induced apoptosis by inhibiting ANT2 in LNCaP cells.

Figure 5

LNCaP cells were transfected with siANT2 or siCtrl and were incubated for 48 hr. A, ANT2 mRNA was quantified by real-time RT-PCR and normalized by GAPDH. B, Protein levels of DR5 and β-actin were analyzed by Western blotting. C, The cells were incubated with or without 50 ng/ml Apo2L/TRAIL. After 24 hr, the sub-G1 population of the cells was measured with flow cytometry. D, The cells were incubated with 20 µmol/L apigenin for 24 hr, followed by treatment with or without 50 ng/ml Apo2L/TRAIL. The Apo2L/TRAIL activity in samples without apigenin was normalized to 1. E, The cells were incubated with 20 µmol/L apigenin for 24 hr. Protein levels of DR5 and β-actin were analyzed by Western blotting. Columns, mean; bars, SD (n = 3). *P<0.05, **P<0.01 relative to control.