Figure 6. TNFα inhibits the association of Snail and GSK3β.

(A) HCT116 cells were treated with TNFα (20 ng/ml) or MG132 (10 µM) for 6 h. After Snail was immunoprecipitated from equal amount of lysates (two lower panels), the ubiquitination of Snail was examined by western blotting. (B) HCT116 cells were treated with TNFα (20 ng/ml) or MG132 (10 µM) for 6 h. Snail or GSK-3β were immunoprecipitated respectively from equal amount of lysates and the associated GSK-3β or Snail were detected by western blotting.