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. 2012 Dec 28;5(3):1053–1057. doi: 10.3892/ol.2012.1098

Figure 1.

Figure 1

Overexpression of glutathione S-transferase π (GSTP1) suppresses epidermal growth factor (EGF)-induced Stat3 activity. (A) HepG2 cells were transfected with the xpress-tagged GSTP1 expression plasmid (0.5, 1 and 2 μg). The cells were either left untreated or treated with EGF (100 ng/ml) for 15 min. Total cell lysates were prepared and subjected to western blot analysis using antibody against phospho-Tyr-705-Stat3 (p-Stat3 Y705) or phospho-Ser-727-Stat3 (p-Stat3S727). (B) GSTP1 suppresses EGF-induced Stat3 promoter activity. The HepG2 cells in 12-well plates were transiently transfected with different quantities of the xpress-tagged GSTP1 expression plasmid together with the pCMV-β-gal control vector and Stat3 reporter plasmid. Twenty-four hours following transfection, the cells were stimulated for 15 min. Cell lysates were prepared to measure luciferase activity using the Luciferase Assay System (Promega Corporation; Madison, WI, USA) and analyzed by the Luminometer TD-20/20 (Turner Biosystems, Inc.; Sunnyvale, CA, USA). Luciferase activity was normalized to β-gal activity.