Figure 3.

Glutathione S-transferase π (GSTP1) overexpression results in a reduction of proliferation and an increase in the number of cells in the G0/G1 phase. The HepG2 cells were transfected with empty vector and wild-type GSTP1 prior to treatment with epidermal growth factor (EGF). The extent of cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In each experiment, averages of eight replicates were normalized to the average of the vector control. Data are presented as mean ± standard error of the mean from ≥3 independent experiments.^*P<0.05 compared with the vector control.