Table 4. Fitness of pyrimorph-resistant mutants (mutant designations begin with the letter R) and the corresponding parental isolates of Phytophthora capsici a.
In vitro b | In planta c | ||||
Isolate | Sporulation (105/cm2) | Cystospore germination (%) | Lesion area (cm2) | Sporulation (105/cm2) | Disease scored |
Hd3 | 25.0a | 73.0b | 5.3a | 5.1b | 2.5a |
R3-1 | 1.7b | 88.0a | 2.4b | 8.7a | 2.3a |
R3-2 | 4.2b | 67.0b | 3.6ab | 0.6c | 0.7b |
R3-3 | 5.0b | 94.0a | 2.5b | 0.6c | 0.4b |
Hd11 | 9.0a | 90.5a | 6.8a | 7.0a | 3.9a |
R11-1 | 3.1b | 79.3b | 5.2b | 3.5b | 4.5a |
R11-2 | 1.3c | 88.3a | 3.4c | 0.5c | 1.1b |
Hx18 | 24.0a | 82.0ab | 9.3a | 7.0a | 2.8a |
R18-1 | 4.3c | 77.0b | 8.7a | 4.5c | 0.6b |
R18-2 | 0.8d | 85.0a | 4.8bc | 0.7d | 1.2b |
R18-3 | 1.7d | 70.5c | 3.5cd | 0.6d | 0.1b |
R18-4 | 0.3d | 77.0b | 2.6d | 4.0c | 0.1b |
R18-5 | 0.4d | 85.0a | 5.0bc | 5.1bc | 0.2b |
R18-6 | 7.0b | 87.0a | 5.4b | 6.4ab | 1.2b |
Dz21 | 13.3a | 85.0a | 8.4a | 6.5a | 3.2a |
R-21 | 19.5a | 72.5b | 6.1b | 5.5a | 1.7b |
Means in a column followed by the same letter are not significantly different at p = 0.05 according to Fisher’s least significant difference.
On carrot agar.
Lesion area and sporulation were determined on detached pepper leaves, and disease score was determined on growing pepper plants.
A disease severity scale of 0–5 was used: 0, no visible symptoms; 1, leaves slightly wilted with black lesions beginning to appear on stems or 10–29% of entire plant diseased; 2, 30–49% of entire plant diseased; 3, 50–69% of entire plant diseased; 4, 70–90% of entire plant diseased; 5, dead plant [37], [38].