Table 1. Primer used by RT-PCR.
| Gene | Name | Direction | Sequence (5–3) | Length of amplicon(bp) | Annealing temp. |
| Elongation factor 1 alfa (Elgfα) | Elgf1α | Fwd | GCTGTGCGTGACATGAGG | 88 | 60 |
| Rev | ACTTTGTGACCTTGCCGC | ||||
| Interferon alfa IFNa-1 | IFNα-1 | Fwd | TGGGAGGAGATATCACAAAGC | 163 | 60 |
| Rev | TCCCAGGTGACAGATTTCAT | ||||
| Mx protein | Mx | Fwd | TGCAACCACAGAGGCTTTGAA | 78 | 60 |
| Rev | GGCTTGGTCAGGATGCCTAAT | ||||
| IgM heavy chain membrane bound form IgM | IgM | Fwd | TCTGGGTTGCATTGCCACTG | 121 | 60 |
| Rev | GTAGCTTCCACTGGTTTGGAC | ||||
| Recombination activating gene-1 | RAG-1 | Fwd | CCTAACACCTCTAGGCTTGAC | 103 | 60 |
| Rev | GCTTCCCTGTTTACTCGC | ||||
| T cell receptor alpha chain | TCAC | Fwd | GCCTGGCTACAGATTTCAGC | 107 | 66 |
| Rev | GGCAACCTGGCTGTAGTAAGC | ||||
| VP1 | LVP1mPCR507 | Fwd | CTGGTCCAGAAACCCTAAGAC | 506 | 60 |
| RVP1mPCR1014 | Rev | GTGTGTATCTCTCCCCTTTTGG | |||
| RT-PCR primers | |||||
| Infectious pancreatic necrosis virus, A segment | IPNV1F | Fwd | ATCTGCGGAGTAGACATCAAAG | 223 | 59 |
| IPNV2R | Rev | TGCAGTTCTTCGTCCATCCC | |||
| Infectious pancreatic necrosis virus, A segment | A-Sp500F | Fwd | CAAGGATGGTATTCACCG | 1189 | 59 |
| A-Sp1689R | Rev | AGCCTGTTCTTGAGGGCTC | |||
This table shows primer sequences for genes tested by quantitivate real-time RT-PCR for mRNA expression. The primers used for RT-PCR for detection of IPNV genome in kidney are given in the two last rows of the table.