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. 2013 Feb 19;8(2):e54656. doi: 10.1371/journal.pone.0054656

Table 2. Virus detection by cell culture and RT-PCR at persistent period (6 months).

Strains 1 month 2 months 3 months 4 months 5 months 6 months
CC RT-PCR CC RT-PCR CC RT-PCR CC RT-PCR CC RT-PCR CC RT-PCR
TA 5/5 Nd 3/5 2/2 0/5 4/5 2/5 3/3 2/5 3/3 5/5 Nd
TT 5/5 Nd 4/5 0/1 0/5 5/5 2/5 3/3 1/5 4/4 5/5 Nd

Result of reisolation by cell culture (CC) or detection of virus genome by reverse transcriptase PCR (RT-PCR). Numbers indicate positive/examined for the two methods. When all fish in one group were positive by cell culture they were not examined by RT-PCR. When fish were negative for cell culture, they were subsequently examined by RT-PCR. Strains indicate IPNV strains used for challenge and time points are time post challenge. Nd-not done.