Table 2. Virus detection by cell culture and RT-PCR at persistent period (6 months).
Strains | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | ||||||||
CC | RT-PCR | CC | RT-PCR | CC | RT-PCR | CC | RT-PCR | CC | RT-PCR | CC | RT-PCR | |||
TA | 5/5 | Nd | 3/5 | 2/2 | 0/5 | 4/5 | 2/5 | 3/3 | 2/5 | 3/3 | 5/5 | Nd | ||
TT | 5/5 | Nd | 4/5 | 0/1 | 0/5 | 5/5 | 2/5 | 3/3 | 1/5 | 4/4 | 5/5 | Nd |
Result of reisolation by cell culture (CC) or detection of virus genome by reverse transcriptase PCR (RT-PCR). Numbers indicate positive/examined for the two methods. When all fish in one group were positive by cell culture they were not examined by RT-PCR. When fish were negative for cell culture, they were subsequently examined by RT-PCR. Strains indicate IPNV strains used for challenge and time points are time post challenge. Nd-not done.