Figure 3.

Cadherin switch in P1 cells induced by cell detachment. (A) Lung cancer cells were detached and suspended in poly-HEMA-coated plates for 24 h, cell lysates were collected and E- and N-cadherin expression was analyzed by immunoblotting. β-actin was used as a control. (B) Effect of cell detachment on E-cadherin expression in various lung cancer cells. Columns represent mean ± SD (n=3), ^*P<0.05, vs. adherent cells. (C) Effect of cell detachment on N-cadherin expression in various lung cancer cells. Columns represent mean ± SD (n=3), ^*P< 0.05, vs. adherent cells. HEMA, poly 2-hydroxyethylmethacrylate.