Table 2.
Absolute numbers of peptide-reactive T cells in the periphery (per 150,000 cells pre-/postvaccination).
| Patient | TYMS | IGF-BP3 | RGS-5 | VEGF | MMP7 | CA IX | PRUNE | TIF1 | NY-ESO | Pool Ca | Pool Eb | DTH |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MR-1 | 51/41c | 43/32 | d−/16 | 45/85 | 14/43e | −/24 | nd/nd | 22/nd | 81/112 | −/15 | 12/51 | + |
| MR-2 | 12/45 | 64/183 | 49/135 | 22/124 | 48/162 | −/65 | −/66 | 50/82 | 24/173 | 19/107 | 39/157 | ++ |
| MR-4 | 23/70 | 38/107 | 123/40 | 80/70 | 76/157 | −/29 | 105/58 | nd/73 | 40/63 | 66/71 | 99/97 | + |
| MR-5 | −/17 | −/60 | 19/41 | 32/41 | 24/42 | −/23 | nd/nd | −/37 | −/64 | −/64 | 13/73 | + |
| MR-6f | 12/27 | 30/48 | 49/18 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/11 | − |
| MR-7f | −/− | −/− | −/50 | −/13 | −/46 | −/− | nd/nd | 13/55 | −/− | 22/126 | 15/46 | + |
| MR-9f | −/− | −/− | −/− | −/− | −/28 | −/− | −/− | −/− | −/12 | 15/31 | 39/37 | (+) |
| MR-10 | 60/20 | 64/13 | 82/49 | 77/33 | 56/45 | 32/− | 32/19 | 89/57 | 69/29 | 69/54 | 123/155 | (+) |
| MR-11 | −/39 | 99/37 | −/43 | 51/nd | −/− | 42/− | −/− | 29/46 | 142/255 | 52/107 | 128/210 | + |
| MR-13 | 28/46 | 71/80 | 40/62 | 61/32 | 111/17 | −/− | −/− | 40/101 | 80/32 | 41/74 | 157/125 | ++ |
| MR-14 | 23/36 | 76/141 | 39/49 | −/126 | −/64 | 28/33 | −/14 | 52/67 | 16/44 | −/63 | 12/70 | ++ |
| MR-15 | 11/− | −/28 | −/15 | −/59 | 15/25 | −/− | −/25 | 4/53 | 14/31 | −/14 | −/67 | + |
DTH, delayed-type hypersensitivity reaction; nd, not determined (not enough cells).
Pool C contains the peptides for ORMDL3, AHNAK-rel, BTG1, PIG10, LENG4, CP and PRAME.
Pool E contains the peptides for TIF1, CLIC1, ELAC2 and TMP1.
The values represent absolute numbers of spots for pre- and postvaccination (pre/post) responses. These values are given after subtraction of background responses (that is, without peptide or with irrelevant Bcr/Abl peptide). The highest postvaccination response is given irrespective of the time point postvaccination (that is, at 6, 14, 22 or 36 wks).
No values mean no reactivity (≤10 spots above background, which corresponds to a frequency of ≤1 = 1.5 × 104 IFN-γ–secreting peptide-specific T cells).
Values representing a greater than two-fold increase in reactivity against the peptides post-versus prevaccination are indicated in bold. With the larger peptide pools, a reactivity of ≥40 spots above background was considered positive.
Short protocol of vaccination starting with the middle dose of 107 vaccine cells. DTH reactivity: −, negative; (+), weak; +, positive; ++, positive (>2 cm redness and/or induration).