Figure 4.

(A) One application cycle of the assay illustrating type of application: buffer type or sample. Flow rate and length of application as indicated (horizontal bars). (B) Examples of corresponding current traces from an application cycle and a test application using an antagonist. First application scale showing buffer changes as in panel A. (Vertical scale bar) Current amplitude. (Horizontal bar) Timescale for all traces. (C) Example current traces from control and test application obtained using the application cycle shown in panels A and B. The current traces are recorded from, and comparing, TRPA1 WT; rat residues substituted in to the human TRPA1 receptor, ratified; and the TRPA1 F944A substitution in control and with 1 μM AZ868 applied as indicated above the traces. The scale bars show current amplitude and timecourse for all three receptor constructs.