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. 2013 Jan 2;20(1):55–66. doi: 10.1093/dnares/dss033

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© The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cell aggregation phenotype of engineered parent strains across QTL genes. Cell aggregation phenotype that was scored by the sedimentary assay (the left panel) and microscope view (the right panel) for the two parental strains and their genetically modified strains through knock out or allele replacement. Red horizontal line in the left panel marks the top boundary of cell sediment. (a) The three QTL genes (AMN1, FLO8, and RGA1) were knocked out in the clumpy parent YL1C. ‘Δ’ represents deletion, i.g. ‘amn1Δ’ for deletion AMN1. (b) The alleles of three QTL genes were replaced in situ by their counterpart alleles between the parents. The superscripts ‘A’ and ‘C’ refer to the YH1A-and YL1C-derived allele, respectively, i.g. AMN1^A represents the allele of AMN1 derived from YH1A.