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. 2013 Jan 2;20(1):55–66. doi: 10.1093/dnares/dss033

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© The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Functional verification of AMN1 underlying yeast cell aggregation. (a) Yeast two-hybrid assay on interaction between the AMN1 and TEM1 genes. (b) Expressional assay of the three downstream genes, DSE1, DSE2, and SCW11, from the two parental strains. (c) The microscopic view and the expression assay of DSE1, DSE2, and SCW11 between two strains derived from YL1C, only differing in the 368th amino acid residue of Amn1. (d) A hypothetical model on how the single nucleotide substitution (V368D) of Amn1 affects the MEN signal transduction pathway, which, in turn, determines daughter cell separation and formation of cell clumps.