TABLE 1.
Results of tumorigenicity determination in athymic nude mice
| Anchorage- Independent Clone |
Cage-Mouse ID |
Tumor at Injection Sites |
Fraction of injection sites with tumor |
Fisher’s exact test P valuea |
|
|---|---|---|---|---|---|
| Left | Right | ||||
| 1 | 1–1 | Yes | Yes | 6/6 (100%) | < 0.0001 |
| 1–2 | Yes | Yes | |||
| 1–3 | Yes | Yes | |||
| 2 | 2–1 | Yes | Yes | 2/2 (100%) | 0.0110 |
| 2–2 | Animals diedb | ||||
| 2–3 | |||||
| 3 | 3–1 | Yes | Yes | 2/2 (100%) | 0.0110 |
| 3–2 | Animals diedb | ||||
| 3–3 | |||||
| 4 | 4–1 | Yes | Yes | 2/6 (33%) | 0.0980 |
| 4–2 | No | No | |||
| 4–3 | No | No | |||
| 5 | 6–1 | Yes | Yes | 4/6 (67%) | 0.0049 |
| 6–2 | Yes | Yes | |||
| 6–3 | No | No | |||
| 6 | 8–1 | Yes | Yes | 4/6 (67%) | 0.0049 |
| 8–2 | Yes | Yes | |||
| 8–3 | No | No | |||
| 7 | 9–1 | Yes | Yes | 4/6 (67%) | 0.0049 |
| 9–2 | Yes | Yes | |||
| 9–3 | No | No | |||
| 8 | 10–1 | Yes | Yes | 6/6 (100%) | < 0.0001 |
| 10–2 | Yes | Yes | |||
| 10–3 | Yes | Yes | |||
| Saline (control) | 5–1 | No | No | 0/12 (0%) | N/A |
| 5–2 | No | No | |||
| 5–3 | No | No | |||
| 7–1 | No | No | |||
| 7–2 | No | No | |||
| 7–3 | No | No | |||
a
Fisher’s exact test was performed to compare the fractions of injection sites with tumors between the control group (injected with saline) and each group of mice injected with cells of anchorage-independent clones derived from HZE particle irradiated HTori-3 cells.
b
Animals died before any tumors became palpable.