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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Biochem Biophys Res Commun. 2012 May 16;422(4):561–567. doi: 10.1016/j.bbrc.2012.05.024

Fig. 4.

Fig. 4

CDDO-Me inhibits hTERT regulatory proteins. (A) MiaPaCa-2 cells were treated with CDDO-Me (0–5 μM) for 48 h and cell lysates were analyzed for c-Myc, Sp1, NF-κB, STAT-3, p-STAT-3, Akt and p-Akt by western blotting (A). hTERT is a target of CDDO-Me. Tumor cells were transfected with siRNA-hTERT (A) or hTERT expression plasmid (pCI-neo-hTERT) (B) for 48 h. hTERT levels were measured by immunoblotting (insets) and response to CDDO-Me was assessed in 48 h MTS assay. Each experiment was repeated two times.

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