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. Author manuscript; available in PMC: 2013 Feb 20.
Published in final edited form as: Nat Cell Biol. 2011 Feb 20;13(3):324–330. doi: 10.1038/ncb2174

Figure 2. Host cells fail cytokinesis.

Figure 2

(a) Graph: % failed mitoses (time-lapse analysis) of MCF10A-H2B-GFP cells (single cells n=1893; cell-in-cell, n=178; p<.001 (Fisher Exact test)), MCF7 cells (single cells n=298, cell-in-cell n=35; p<.001), and 16HBE cells (single cells n=1277, cell-in-cell n=36; p<.001). Images show failed divisions of MCF10A (top row) and 16HBE (bottom row). Left images: mononucleate (arrow) cell-in-cell structures; Middle: mitosis initiated by host cells; Right: binucleate (arrows) host cells after cytokinesis failure. See Movies S1 and S2. (b) Internalized cells do not disrupt spindle formation. Graph: prophase to anaphase timing for single cells (left, n=22) or host cells (right, n=22), determined by time-lapse of GFP-Tubulin-expressing MCF10A cells; p=0.41 (Mann-Whitney). Data points show individual mitoses timed from spindle formation to anaphase, bar represents mean. Red data points are failed divisions. Representative images from successful (1,2) and failed (3) host cell divisions are shown. See Movies S3–S5. Bars = 10μm.