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Comparison of EGFR T790M inhibitors in human NSCLC-derived cell lines. A, NCI-H1975 (EGFR L858R/T790M), B, NCI-H820, or C, PC-9 ER (del E746_A750/T790M) cells were treated with the indicated inhibitors for 16 hr and stimulated by TGF-α for 15 min. The drug effects on EGFR phosphorylation were observed by immunoblotting and an in-cell immunodetection assay as described in Fig. 4.
