Table 1.
Tet(O) variant | Tetracycline MIC (μg/ml) | % inhibitiona |
---|---|---|
wt | 2 | 0 |
L466A | 1.75 | 33 |
P438A | 0.75 | 83 |
Y507A | 0.75 | 83 |
S472A | 1 | 67 |
VPP436-438G | 0.5 | 100 |
LGY466-468G | 0.5 | 100 |
YSP507-509G | 0.5 | 100 |
BW25993 E. coli lacking the plasmid-expressed Tet(O) | 0.5 | 100 |
Percentage of inhibition was calculated as (MIC(Tet(O)) − MIC(Tet(O) mutant))/(MIC(Tet(O) − MIC(BW))×100