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. Author manuscript; available in PMC: 2013 Feb 20.
Published in final edited form as: Mucosal Immunol. 2010 Dec 22;4(4):397–408. doi: 10.1038/mi.2010.82

Figure 4.

Figure 4

IL-13 expression and goblet cell analysis. (a–c) Immunohistochemical staining for interleukin 13 (IL-13; red, in a) and IL-13 receptor α1 (IL-13Rα1; red, in b) and goat anti-rabbit secondary antibody alone (c) in conjunctiva sections of nonstressed (NS) control mice. Scale bar = 25 μm. (d) mRNA transcript levels of IL-13 and interferon-γ (IFN-γ) in conjunctiva in NS control mice and mice subjected to desiccating stress for 5 or 10 days (DS5 and DS10, respectively). (e) Ratio of IL-13/IFN-γ mRNA transcripts in conjunctiva. (f) Mean±s.d. of goblet cell (GC) density in NS C57BL/6 (B6) control mice and mice subjected to DS5 and mice after subconjunctival injection of BSA (+ BSA) or IL-13 (+ IL13). (g) Mean±s.d. of GC density in NS C57BL/6 (B6) controls mice and mice subjected to DS5 and IL-13 knockout (KO) mice. (h) Mean±s.d. of GC density in NS Bal/c (BC) control mice and mice subjected to DS5 and DS10 and signal transducer and activator of transcription 6 knockout (STAT6KO) at baseline and after DS5. (i) Mean±s. d. of GC density in NS C57BL/6 mice (B6) that received systemic injection of neutralizing antibody (NK1.1) to natural killer (NK) cells (αNK) or isotype control (IC) antibody before NS and after DS5. (j) Mean±s.d. of GC density in NS C57BL/6 (B6) control and RAG1KO mice. (k) Mean±s.d. of GC density in NS Bal/c (BC) control mice and CD1dKO at baseline, NS, and after DS5. A separate group NS CD1dKO mice received systemic injection of depleting antibody (NK1.1) to NK cells (αNK) or IC antibody.