Figure 2.

Effect of cilostazol on cellular senescence in articular chondrocytes. (A) Rat articular chondrocytes were treated with 50 μM cilostazol or 200 μg/ml etoposide for 24 h (■, total cells; □, apoptotic cells). The cells were assessed for apoptosis by TUNEL assay. TUNEL-positive cells were counted under a confocal microscope; 250–300 cells were counted for each condition. Three independent experiments were performed, and values are represented as means ± SD from the 3 experiments. (B) Rat chondrocytes were treated with different concentrations of cilostazol for 48 h and stained for SA-β-gal activity; representative images were obtained at ×20 magnification. Percentages of SA-β-gal-positive cells were determined from the numbers of blue cells per 200 cells in a randomly selected area.