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. Author manuscript; available in PMC: 2013 Feb 20.
Published in final edited form as: Am J Respir Cell Mol Biol. 2011 Feb 4;45(4):746–752. doi: 10.1165/rcmb.2010-0304OC

Figure 6.

Figure 6

Effect of the H2S donor, NaSH, on FCS-induced activation of extracellular signal–regulated kinase (ERK)–1/2 and p38 mitogen-activated protein kinase (MAPK) in human ASM cells. NaSH inhibited FCS-induced ERK-1/2 and p38 phosphorylation. ASM cells were incubated with methemoglobin (10 μM) for 1 hour; NaSH (100 μM) was added for another 48 hours. Total and phospho–ERK-1/2, total and phospho-p38 and β-actin were detected by Western blotting (A). (B) Changes in phospho-MAPK expression were quantitated by densitometry, normalized against β-actin expression, and then expressed as the percent change versus nonphosphorylated controls. Bars represent means (±SEM) of six ASM donors. **P < 0.01. P-ERK, phosphorylated-ERK; P-p38, phosphorylated-p38; Stim, stimulated.