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. 2011 Dec 29;29(4):541–549. doi: 10.3892/ijmm.2011.875

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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ER-400583-00 suppressed cell proliferation distal to the region of blood perfusion in U251 xenograft tumors. Mice bearing U251 tumors were orally treated with either vehicle or 100 mg/kg ER-400583-00 once daily for 3 days. Tumors were collected 24 h after the last treatment. Hoechst 33342 fluorescent images of tumor cryosections were digitally captured, and overlaid onto images from subsequent immunohistochemical staining for BrdU. (A) Representative composite image of a vehicle control tumor and (B) an ER-400583-00-treated tumor. Hoechst 33342 is shown in blue and BrdU in brown. Bar, 200 μm. (C) The fraction of BrdU labeling at each distance from the nearest region of blood perfusion. Entire tumor sections were analyzed. Data points represent the mean of four tumors; bars represent the SD. (D) The ratio of the BrdU-positive fraction in the ER-400583-00-treated tumors relative to the vehicle control tumors. Data points represent the mean of four tumors; bars represent the SD.

ER-400583-00 suppressed cell proliferation distal to the region of blood perfusion in U251 xenograft tumors. Mice bearing U251 tumors were orally treated with either vehicle or 100 mg/kg ER-400583-00 once daily for 3 days. Tumors were collected 24 h after the last treatment. Hoechst 33342 fluorescent images of tumor cryosections were digitally captured, and overlaid onto images from subsequent immunohistochemical staining for BrdU. (A) Representative composite image of a vehicle control tumor and (B) an ER-400583-00-treated tumor. Hoechst 33342 is shown in blue and BrdU in brown. Bar, 200 μm. (C) The fraction of BrdU labeling at each distance from the nearest region of blood perfusion. Entire tumor sections were analyzed. Data points represent the mean of four tumors; bars represent the SD. (D) The ratio of the BrdU-positive fraction in the ER-400583-00-treated tumors relative to the vehicle control tumors. Data points represent the mean of four tumors; bars represent the SD.