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. 2013 Feb 11;14:34. doi: 10.1186/1471-2369-14-34

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Copyright ©2013 Miyakawa et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PC2 trafficking to the PM in human proximal tubule (hPT) cells. (A-F). Immunocytochemistry of PC2 in hRT cells treated with control (A), 1 μM ouabain (C), or 25 μM ATP (E). Scale bars, 20 μm. Localization profile of PC2 along a line (as indicated) through the center of a single hPT cell treated with control (B), ouabain (D), or ATP (F). Gray areas represent PM regions. (G) Quantitative analysis of a number of PM PC2-positive hRT cells following indicated treatments. Values are the mean ± SEM, and ***P < 0.001 vs. control.