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. 2013 Jan 10;10:6. doi: 10.1186/1743-7075-10-6

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Copyright ©2013 Mani et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of increasing porcine bile acid concentration on ex vivo intestinal integrity and permeability. A) Transepithelial resistance (TER) and B) FITC-Dextran transport (4.4 kDa). Freshly isolated ileum samples were mounted into modified Ussing chambers and incubated with the indicated concentration of bile acid for 30 minutes and then FITC-Dextran was added to mucosal side. Permeation coefficient was calculated by taking samples from chambers every 10–15 minutes and measuring the amount of fluorescence. Different letters represent significant difference at P < 0.05. n = 11 pigs. Data are means ± S.E.M.