Table 4.
Specificity of anti-CSP repeats ELISA using sera pre-incubated with different antigens
| Sample Code |
Concentration of anti-CSP repeats antibody (EU/ml) Inhibited with: |
% inhibition |
|||||
|---|---|---|---|---|---|---|---|
| R32LR | AMA-1 | HBsAg | CONTROL* | R32LR | AMA-1 | HBsAg | |
| 1 |
37 |
70 |
69 |
76 |
51.3 |
7.9 |
9.2 |
| 2 |
80 |
134 |
141 |
138 |
42.0 |
2.9 |
−2.2 |
| 3 |
56 |
97 |
102 |
105 |
46.7 |
7.6 |
2.9 |
| 4 |
97 |
156 |
160 |
162 |
40.1 |
3.7 |
1.2 |
| 5 |
146 |
249 |
243 |
250 |
41.6 |
0.4 |
2.8 |
| 6 |
51 |
89 |
98 |
97 |
47.4 |
8.2 |
−1.0 |
| 7 |
84 |
148 |
167 |
167 |
49.7 |
11.4 |
0.0 |
| 8 |
124 |
213 |
223 |
217 |
42.9 |
1.8 |
−2.8 |
| 9 |
121 |
222 |
235 |
222 |
45.5 |
0.0 |
−5.9 |
| Mean | 45.2 | 4.9 | 0.5 | ||||
AMA-1, apical membrane antigen-1; CSP, circumsporozoite protein; HBsAg, hepatitis B surface antigen.
* control: no inhibitory antigen used (co-incubation with diluent).