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. 2012 Apr 24;13:149. doi: 10.1186/1471-2164-13-149

Table 1.

Summary of the numbers of contigs retained in each step when characterizing the transcriptome of Paradoxornis webbianus bulomachus

Step
Reference-guided assembly
De novo assembly
Total
  Zebra Finch Chicken    
Assembly
14,215
7,508
465,613
487,336
Coverage filtered1
10,076
680
57,035
67,791
BLASTed
9,923
528
16,028
26,479
Of which:
 
 
 
 
Homology filtered2
6,359
31
1,389
7,779
GO annotated 5,426 50 889 6,365

1 Contigs filtered with coverage of > 10 X.

2 Contigs filtered with similarity (≥ 90%) and alignment length (≥ 200 amino acid residues) with their best matches used to analyze the top-hit species distribution.