Table 2.
Distribution of BLASTx top-hit species of Paradoxornis webbianus bulomachus contigs with a similarity of > 90% and an alignment length of > 200 amino acid residues to their best-hit sequences
|
Category Species |
Reference-guided assembly |
|
|
|
|---|---|---|---|---|
| |
Zebra Finch |
Chicken |
De novo assembly |
Total |
| No. of contigs (%) | No. of contigs (%) | No. of contigs (%) | No. of contigs (%) | |
|
Aves |
6159 (83.53) |
27 (0.35) |
936 (12.03) |
7122 (91.55) |
|
Taeniopygia guttata |
5141 (66.09) |
|
401 (5.15) |
5542 (71.24) |
|
Gallus gallus |
726 (9.33) |
20 (0.26) |
485 (6.23) |
1231 (15.82) |
|
Meleagris gallopavo |
257 (3.30) |
6 (0.08) |
38 (0.49) |
301 (3.87) |
|
Zonotrichia albicollis |
20 (0.26) |
1 (0.01) |
5 (0.06) |
26 (0.33) |
|
Coturnix coturnix |
5 (0.06) |
|
3 (0.04) |
7 (0.09) |
| Other avian species1 |
10 (0.13) |
|
4 (0.05) |
15 (0.19) |
|
Mammals |
165 (2.12) |
4 (0.05) |
316 (4.06) |
485 (6.23) |
|
Monodelphis domestica |
27 (0.35) |
|
67 (0.86) |
94 (1.21) |
|
Homo sapiens |
28 (0.36) |
2 (0.03) |
26 (0.33) |
56 (0.72) |
|
Mus musculus |
22 (0.28) |
|
33 (0.42) |
55 (0.71) |
|
Ailuropoda melanoleuca |
6 (0.08) |
|
27 (0.35) |
33 (0.42) |
|
Ornithorhynchus anatinus |
10 (0.13) |
|
22 (0.28) |
32 (0.41) |
|
Equus caballus |
5 (0.06) |
|
22 (0.28) |
27 (0.35) |
|
Bos taurus |
5 (0.06) |
|
20 (0.26) |
25 (0.32) |
|
Sus scrofa |
3 (0.04) |
|
16 (0.21) |
19 (0.24) |
|
Macaca mulatta |
5 (0.06) |
|
13 (0.17) |
18 (0.23) |
|
Rattus norvegicus |
4 (0.05) |
|
14 (0.18) |
18 (0.23) |
|
Oryctolagus cuniculus |
2 (0.03) |
|
13 (0.17) |
15 (0.19) |
|
Pongo abelii |
4 (0.05) |
|
9 (0.12) |
13 (0.17) |
|
Callithrix jacchus |
5 (0.06) |
|
8 (0.10) |
13 (0.17) |
|
Loxodonta africana |
7 (0.09) |
1 (0.01) |
2 (0.03) |
10 (0.13) |
|
Canis familiaris |
1 (0.01) |
|
9 (0.12) |
10 (0.13) |
|
Cricetulus griseus |
5 (0.06) |
|
3 (0.04) |
8 (0.10) |
| Other mammalspecies2 |
26 (0.33) |
1 (0.01) |
12 (0.15) |
39 (0.50) |
|
Reptiles |
19 (0.24) |
|
32 (0.41) |
51 (0.66) |
|
Anolis carolinensis |
8 (0.23) |
|
28 (0.36) |
46 (0.59) |
| Other reptile species3 |
1 (0.01) |
|
4 (0.05) |
5 (0.06) |
|
Amphibians |
10 (0.13) |
|
19 (0.24) |
29 (0.37) |
|
Xenopus tropicalis |
7 (0.09) |
|
15 (0.19) |
22 (0.28) |
|
Xenopus laevis |
3 (0.04) |
|
4 (0.05) |
7 (0.09) |
|
Fish |
6 (0.08) |
|
8 (0.10) |
14 (0.18) |
|
Danio rerio |
|
|
5 (0.06) |
5 (0.06) |
|
Tetraodon nigroviridis |
1 (0.01) |
|
2 (0.03) |
3 (0.04) |
|
Salmo salar |
1 (0.01) |
|
1 (0.01) |
2 (0.03) |
|
Cyprinus carpio |
3 (0.04) |
|
|
3 (0.04) |
|
Insect |
|
|
|
|
|
Danaus plexippus |
1 (0.01) |
|
|
1 (0.01) |
|
Protozoa |
|
|
38 (0.49) |
38 (0.49) |
|
Trichomonas vaginalis |
|
|
38 (0.49) |
38 (0.49) |
|
Plants |
|
|
36 (0.46) |
36 (0.46) |
|
Zea mays |
|
|
33 (0.42) |
33 (0.42) |
| Other plant species4 |
|
|
3 (0.04) |
3 (0.04) |
|
Others5 |
|
|
4 (0.05) |
4 (0.05) |
| Total | 6359 (87.75) | 31 (0.40) | 1389 (17.86) | 7779 (100) |
Totals for each category are in bold.
1 Other avian species included Anas platyrhynchos, Anser anser, Columba livia, Dromaius novaehollandiae, Lonchura striata, and Struthio camelus.
2 Other mammal species included Cavia porcellus, Felis catus, Heterocephalus glaber, Macaca fascicularis, Mustela putorius furo, Nomascus leucogenys, Pan troglodytes, and Serinus canaria.
3 Other reptile species included Alligator mississippiensis, Iguana iguana, Psammobates geometricus, and Pseudemys nelson.
4 Other plant species included Oryza sativa, Sorghum bicolor, and Vitis vinifera.
5 Others included sequences of unknown species and synthetic constructs.