Figure 1.

The regulation of gene expression during germination encompasses a large number of genes and distinct regulatory programs. (A) Experimental setup. Dormant spores were inoculated in pre-warmed YPD and allowed to germinate at 30°C under vigorous shaking. Samples were taken at the indicated time points (blue arrows), and mRNA expression levels throughout the time course were compared to that of dormant spores. (B) Genes that showed at least two fold altered expression in two or more consecutive time points during the germination process were subjected to K-means clustering and grouped in eight clusters. The right and lower panels display the (C) average expression profile for each cluster (error bars indicate standard deviation) and (D) the number of genes and enriched GO terms in each cluster.