Figure 6. Effects of chronic opioid exposure on CA enzyme expression in neonatal rat AMCs.

A, Western blot analysis showing expression of CA isoforms CAI and CAII in 7-day-old cultures of control untreated AMCs versus AMCs treated with combined μ-, δ- and κ-opioid agonists (2 μm); β-actin was used as an internal control. Note the downregulation in CAI and CAII expression in neonatal AMCs following chronic opioid exposure. Values are represented as mean ± SEM of three independent experiments (*P < 0.05).