Table 2.
Solutions
| Components (mm) | I (Ca-free Hank's) | II (CaPSS) | III (K-rich) | IV (Cs-rich) | V (high-K) |
|---|---|---|---|---|---|
| NaCl | 125 | 135 | |||
| CsCl | 135 | ||||
| KCl | 5.36 | 5 | 30 | 150 | |
| MgCl2 | 1.2 | ||||
| CaCl2 | 2 | 3.15 | 3.15 | ||
| Hepes | 11 | 10 | 10 | 10 | 10 |
| Glucose | 10 | 10 | 10 | 10 | 10 |
| Sucrose | 2.9 | ||||
| NaHCO3 | 15.5 | ||||
| Na2HPO4 | 0.336 | ||||
| K2HPO4 | 0.44 | ||||
| K aspartate | 110 | ||||
| CPD | 2.5 | 2.5 | |||
| MgATP | 3 | 3 | |||
| Na2GTP | 0.1 | 0.1 | |||
| EGTA | 5 | 5 | 5 |
Various intracellular free Ca2+ concentrations (10 nm to 1 μm) were used in solution III and V by adjusting the concentrations of EGTA and CaCl2 calculated with Maxchelator software (http://maxchelator.stanford.edu). pH 7.4 for solution II, pH 7.2 for other solutions.