Table 3.
Buffering properties of Casq1-null cells
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
|---|---|---|---|---|---|---|---|---|---|---|
| Pk flux | Amount | [Ca2+]SR | [Ca2+]SR | Δ[Ca2+]SR | BON | Amount | [Ca2+]SR | Δ[Ca2+]SR | ||
| (mm s−1) | ON (μm) | init (μm) | end (μm) | ON (μm) | OFF (μm) | end OFF (μm) | OFF (μm) | BOFF | ||
| BAPTA | 132b | 800 | 529 | 102b | −399 | 36a | −392 | 415 | 347b | 20b |
| (n = 21) | 26 | 68 | 50 | 18 | 57 | 4.5 | 58 | 40 | 44 | 3.3 |
| EGTA | 32 | 1046 | 330 | 36 | −294 | 49 | −543 | 194 | 159 | 50 |
| (n = 8) | 5 | 151 | 59 | 8 | 55 | 5.8 | 122 | 47 | 41 | 7.5 |
| All | 103 | 868c | 474 | 78c | −368c | 40a,c | −434 | 354 | 295c | 28c |
| (n = 29) | 20 | 66 | 43 | 14 | 44 | 3.6 | 54 | 36 | 37 | 4.0 |
Results were determined with D4cpv-based biosensors in individual fibres, then averaged and listed as described for Table 1.
a
Significantly greater than corresponding value at the OFF.
b
Significantly different from corresponding value in solution with 10 mm EGTA.
c
Significantly different from corresponding value in the wild-type.