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. 2012 Nov 19;591(Pt 3):609–625. doi: 10.1113/jphysiol.2012.238014

Figure 1. Silencing efficiency.

Figure 1

A and B, effect of raptor silencing on raptor protein expression and mTORC1 activity. A, representative western blots of raptor (n= 6), phosphorylated S6 kinase (Thr-389) (n= 5), total S6 kinase (n= 6), phosphorylated 4EBP-1 (Thr-37/46) (n= 4), total 4EBP-1 (n= 4), phosphorylated S6 ribosomal protein (Ser-235/236) (n= 4) and total S6 ribosomal protein (n= 4) expression in cell lysates of scramble control, scramble siRNA and raptor silenced cells. Equal loading was performed. B, summary of the western blot data. Values are given as means + SEM. Means without a common letter are statistically different by one-way ANOVA with Tukey–Kramer multiple comparisons post hoc test (P < 0.05). C and D, effect of rictor silencing on rictor protein expression and mTORC2 activity. C, representative western blots of rictor (n= 6), phosphorylated Akt (Ser-473) (n= 3) and total Akt (n= 3) expression in cell lysates of scramble control, scramble siRNA and rictor silenced cells. Equal loading was performed. D, summary of the western blot data. Values are given as means + SEM. Means without a common letter are statistically different by one-way ANOVA with Tukey–Kramer multiple comparisons post hoc test (P < 0.05).