Table 1.
Polymerase chain reaction (PCR) primers.
| Pathogen | Gene target | PCR primers | Size of product (basepairs) | Reference |
|---|---|---|---|---|
| EPEC | eae | 5'-TTATggAACggCAgAggT-3' 5'-CTTCTgCgTACTgCgTTCA-3' |
790 | 13 |
| ETEC | elt | 5'-TCTATgTgCATACggAgC-3' 5'-ATACTgATTgCCgCAAT-3' |
322 | 14 |
| est | 5'-TAAACAAgTAgAggTCTTCAAAA-3' 5'-CggTACAgAgCAggATTACAACA-3' |
147 | 14 | |
| STEC | stx | 5'-ACgAAATAATTTATATgT-3' 5'-TgATTgTTACAgTCAT-3' |
900 | 15 |
| EAEC | pCVD432 | 5'-ggCgAAAgACTgTATCAT-3' 5'-ATgTATAgAAATCCgCTgTT-3' |
630 | 16 |
| EIEC | ial | 5'-CTggTAggTATgCTgAgg-3' 5'-CCAggCCAACAATTATTTCC-3' |
320 | 17 |
ETEC (enterotoxigenic Escherichia coli); EAEC (enteroaggregative E. coli); EPEC (enteropathogenic E. coli); EIEC (enteroinvasive E. coli). Attaching and effacing locus (eae); Heat-labile toxin (LT); Heat-stable toxin (ST); Shiga toxin (stx); EAEC-associated plasmid pCVD432; Invasion-associated locus (ial).