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. 2013 Feb 20;8(2):e56888. doi: 10.1371/journal.pone.0056888

Figure 3. Detection of TLR2 by Western blot in HT-29 cells infected with C. concisus strains.

Figure 3

HT-29 cells were lysed following incubation with C. concisus strains for 24 hours. Expression of TLR2 in HT-29 cells was detected by Western blot. Two bands, the Glycosylated TLR 2 (Gly-TLR4) and non-glycosylated TLR2 (Non-Gly-TLR4), were revealed on Western blot. The levels of Gly-TLR2 and Non-Gly-TLR2 of each sample were expressed as the fold change of the band intensity relative to the band intensity of the negative control (HT-29 cells without C. concisus infection), after normalization to the intensity of the internal control α-Tubulin of the same sample. A: Representative Western blot of Gly-TLR2 (100 kD), Non-Gly-TLR2 (90 kD) and α-Tubulin (55 kD). B: Level of Gly-TLR2 (blue column) and Non-Gly-TLR2 (red column) induced by different C. concisus strains; data were the average of triplicate experiments ± standard error. N: negative control. H2O1-H6O1: C. concisus strains isolated from healthy controls. The remaining C. concisus strains were from patients with IBD. The average levels of Gly-TLR2 and Non-Gly-TLR2 induced by C. concisus strains from patients with IBD were not significantly different that induced by C. concisus strains from healthy controls (P>0.05).