Figure 7. Confocal microscope image of TLR4, MD-2, TLR2, TLR5 and COX-2 expression in HT-29 cells with and without C. concisu infection.

HT-29 cells were incubated with a representative C. concisus strain (P1CDB1) for 24 hours. Expression of TLR4, MD-2, TLR2, TLR5 and COX-2 in HT-29 cells with and without C. concisus infection were detected by immunostained using specific antibodies, followed by Alex Fluor conjugated secondary antibodies. The image was viewed using an Olympus FluoView FV1000 Confocal laser scanning microscope. The secondary antibodies used for detection of TLR4 and MD-2 were conjugated with Alexa Fluor 594 (emission colour red). The secondary antibodies used for detection of TLR2, TLR5 and COX-2 were conjugated with Alexa Fluor 488 (emission colour green). Scale Bar = 10 µm.