Table 3. Yeast strains used in this study.
Strain | Relevant Genotypea | Source/Comments | |
YEF473* | a/α | his3- Δ 200/his3- Δ 200 leu2- Δ 1/leu2- Δ 1 lys2-801/lys2-801 trp1- Δ 63/trp1- Δ 63 ura3-52/ura3-52 | [54] |
YEF1233* | a/α | rga1 Δ ::HIS3/rga1 Δ ::HIS3 | [34] |
YHH415* | a/α | bud8- Δ 1::TRP1/bud8- Δ 1::TRP1 | [20] |
HPY2353* | a/α | CDC3-GFP::LEU2/CDC3-GFP::LEU2 GIC2-PBD-RFP::URA3/GIC2-PBD-RFP::URA3 | This study |
HPY2204* | a/α | rga1 Δ ::HIS3/rga1 Δ ::HIS3 CDC3-GFP::LEU2/CDC3-GFP::LEU2 GIC2-PBD-RFP::URA3/GIC2-PBD-RFP::URA3 | This study |
HPY2370* | a/α | bud8- Δ 1::TRP1/bud8- Δ 1::TRP1 CDC3-GFP::LEU2/CDC3-GFP::LEU2 GIC2-PBD-RFP::URA3/GIC2-PBD-RFP::URA3 | This study |
HPY2371* | a/α | rga1 Δ ::HIS3/rga1 Δ ::HIS3 bud8- Δ 1::TRP1/bud8- Δ 1::TRP1 CDC3-GFP::LEU2/CDC3-GFP::LEU2 GIC2-PBD-RFP::URA3/GIC2-PBD-RFP::URA3 | This study |
HPY2385* | a/α | rga1 Δ ::HIS3/rga1 Δ ::HIS3 bud8- Δ 1::TRP1/bud8- Δ 1::TRP1 | This study |
HPY1680@ | a/α | his3- Δ 1/his3- Δ 1 leu2 Δ 0/leu2 Δ 0 met15 Δ 0/met15 Δ 0 ura3 Δ 0/ura3 Δ 0 | Diploid of BY4741 (Open Biosystems) |
HPY2205@ | a/α | rga1 Δ ::kanMX4/rga1 Δ ::kanMX4 | This study |
HPY2246@ | a/α | rga2 Δ ::kanMX4/rga2 Δ ::kanMX4 | This study |
HPY2384@ | a/α | bem2 Δ ::kanMX4/bem2 Δ ::kanMX4 | This study |
HPY2426@ | a/α | bem3 Δ ::KanMX4/bem3 Δ ::KanMX4 | This study |
Strains marked with *are isogenic to YEF473 and strains marked with @are isogenic to HPY1680 except as indicated.