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. 2013 Feb 20;8(2):e56900. doi: 10.1371/journal.pone.0056900

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© 2013 Iwata et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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C. jejuni NCTC 11168 wild-type strain and its mutants were grown in chicken jejunal extract (A), chicken ileal extract (B), chicken jejunal extract pretreated with cholestyramine (C), and chicken ileal extract pretreated with cholestyramine (D). C. jejuni 81-176 wild-type strain and its mutants were also grown in chicken jejunal extract (E), chicken ileal extract (F), chicken jejunal extract pretreated with cholestyramine (G), and chicken ileal extract pretreated with cholestyramine (H). Each point represents the mean value obtained from triplicate wells in 96-well microtiter plates. The detection limit for the assay was 0.4 log₁₀ CFU/ml.