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. 2013 Feb 20;8(2):e56900. doi: 10.1371/journal.pone.0056900

Table 1. Plasmids and bacterial strains used in this study.

Plasmid or strain Description Source or reference
Plasmids
pGem-T Easy PCR cloning vector, Ampr Promega
pUOA18 E. coli-C. jejuni shuttle vector, Cmr [38]
pUOA18Km pUOA18 modified to replace cat with kan This study
pUOA18Km-hldE pUOA18Km with hldE (cj1150) This study
pUOA18Km-hldD pUOA18Km with hldD (cj1151) This study
pUOA18Km-waaC pUOA18Km with waaC (cj1133) This study
pUOA18Km-waaF pUOA18Km with waaF (cj1148) This study
pUOA18Km-cj1135 pUOA18Km with cj1135 This study
pUOA18Km-cj1136 pUOA18Km with cj1136 This study
pUOA18Km-cj1138 pUOA18Km with cj1138 This study
pUOA18Km-cjj1152 pUOA18Km with cjj1152 This study
pUOA18Km-cjj1165 pUOA18Km with cjj1165 This study
pRK2013 helper plasmid for triparental mating, Kmr [41]
Strains
 C. jejuni parental strains
 NCTC 11168 Wild type; human isolate [28]
 81-176 Wild type; human isolate [55]
 11-164 Wild type; chicken isolate, Nalr This study
C. jejuni mutant strains
 168gmhA NCTC 11168 derivative; gmhA mutant This study
 168hldE NCTC 11168 derivative; hldE mutant This study
 168hldD NCTC 11168 derivative; hldD mutant This study
 168waaC NCTC 11168 derivative; waaC mutant This study
 168waaF NCTC 11168 derivative; waaF mutant This study
 168cj1135 NCTC 11168 derivative; cj1135 mutant This study
 168cj1136 NCTC 11168 derivative; cj1136 mutant This study
 168cj1138 NCTC 11168 derivative; cj1138 mutant This study
 168kpsS NCTC 11168 derivative; kpsS mutant This study
 168hldEc 168hldE/pUOA18Km-hldE This study
 168hldDc 168hldD/pUOA18Km-hldD This study
 168waaCc 168waaC/pUOA18Km-waaC This study
 168waaFc 168waaF/pUOA18Km-waaF This study
 168cj1135c 168cj1135/pUOA18Km-cj1135 This study
 168cj1136c 168cj1136/pUOA18Km-cj1136 This study
 168cj1138c 168cj1138/pUOA18Km-cj1138 This study
 817gmhA 81-176 derivative; gmhA mutant This study
 817hldE 81-176 derivative; hldE mutant This study
 817hldD 81-176 derivative; hldD mutant This study
 817waaC 81-176 derivative; waaC mutant This study
 817waaF 81-176 derivative; waaF mutant This study
 817cjj1152 81-176 derivative; cjj1152 mutant This study
 817cjj1165 81-176 derivative; cjj1165 mutant This study
 817kpsS NCTC 11168 derivative; kpsS mutant This study
 817hldEc 817hldE/pUOA18Km-hldE This study
 817hldDc 817hldD/pUOA18Km-hldD This study
 817waaCc 817waaC/pUOA18Km-waaC This study
 817waaFc 817waaF/pUOA18Km-waaF This study
 817cjj1152c 817cjj1152/pUOA18Km-cjj1152 This study
 817cjj1165c 817cjj1165/pUOA18Km-cjj1165 This study
 164hldE 11-164 derivative; hldE mutant This study
 164hldD 11-164 derivative; hldD mutant This study
 164waaC 11-164 derivative; waaC mutant This study
 164waaF 11-164 derivative; waaF mutant This study
 164hldDc 164hldD/pUOA18Km-hldD This study
E. coli DH5α cloning strain Takara