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Ctrl-KM and k3-KM cells were allowed to adhere to iC3b-coated TC dishes in the presence of mAb KIM185 (10 µg/ml). (A), (C) and (E) are immunoblots of cell lysates for Rac1, Cdc42 and RhoA, respectively. (B), (D) and (F) are pull-down experiments using cell lysates and RBD or PBD-conjugated beads. IB: immunoblotting. A representative experiment from two independent experiments is shown.
