Table 8. Comparison of gene expression level by quantitative real-time PCR and microarray analysis.
N315 ORF | Gene name# | Product | Fold change | |
Real-time PCR | Microarray | |||
SA1029 | ftsZ(3) | Cell division protein | −2.2 | −4.8 |
SA2253 | opp-1C(1) | Oligopeptide transporter permease | +3.4 | +2.1 |
SA0198 | oppF(3) | Oligopeptide transport ATP-binding protein | +3.4 | +2.2 |
SA1287 | asnC(1) | Asparaginyl-tRNA synthetase | −7.4 | −2.4 |
SA1901 | fabZ(3) | (3R)-hydroxymyristoyl ACP dehydratase | −3.1 | −3.7 |
SA2057 | fmhB(1) | FmhB protein | −2.1 | −2.2 |
SA1283 | pbp2(3) | Penicillin-binding protein 2 | −3.1 | −4.5 |
SA0058 | ccrA(2) | Cassette chromosome recombinase A | +2.7 | +2.4 |
SA0039 | mecR1(2) | Methicillin resistance protein | +5.9 | +2.1 |
Treatment of bacterial cell with pentacyclic triterpenoids:
α-amyrin,
betulinic acid,
betulinaldehyde.