Figure 8. PTZ-application promotes phosphorylation of synapsin domain A and B in a dose-dependent fashion.

(A,B) Representative acquisitions of B2 varicosities expressed in pseudocolour, from purple (scarcely stained) via green (weakly stained) to red (most strongly stained), to better appreciate differences in synapsin phosphorylation. Treatments with 20 mM KCL and 40 mM PTZ were shown. Scale bars: 2 μm. (C,D) Quantification of synapsin phospho-levels from varicosities of B2 neurons processed with the indicated treatments and immunolabelled with either α-P₁helSyn or α-P_4,5helSyn antibodies. A ratio between the intensities of varicosity and its proximal neurite has been calculated. The corresponding number of examined varicosities (n) is indicated on each bar.