Figure 9. PTZ-induced epileptiform activity promotes a prolonged phosphorylation of synapsin domain A and B.

(A,B) Pseudocolour representative acquisitions of B2 varicosities and neurites fixed before 40 mM PTZ treatment and every 15 minutes after drug washout. Cells were then immunolabelled with either α-P₁helSyn or α-P_4,5helSyn antibodies, as indicated. Scale bars: 2 μm. (C,D) Bar graphs showing the mean varicosity/neurite fluorescence ratio for synapsin phospho-levels measured as previously reported. A statistically significant increase in synapsin phosphorylation at both phospho-sites was observed even 30 minutes after PTZ washout. The corresponding number of examined varicosities (n) is indicated on each bar.