Figure 5. Enzyme treatment decreases the size of the CD44-positive population.

Following isolation with trypsin, Accutase or enzyme-free (EF) buffer, CA1 cells were subject to FACS analysis either unstained, stained with an isotype control, or stained for CD44. Unstained and isotype-stained cells were unaffected by method of isolation but marked differences in the proportion of cells classified as staining positive for CD44 are seen.