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. 2013 Feb 20;8(2):e57340. doi: 10.1371/journal.pone.0057340

Figure 1. Virus replication is correlated with cyclin E overexpression.

Figure 1

WI-38 or A549 cells were infected with AdGFP, Adwt or Adhz63 at an MOI of 5. (A) Cells were collected at 48 h and then analyzed by Western blot. Cell lysates were immunoblotted for cyclin E and actin. Actin was used as a loading control. (B) CPE was photographed at 72 h post infection (p.i.). All microscopy was originally at a magnification of x100. (C) Viral titers were determined at 72 h p.i. with the infection unit method. The value indicates the mean of three independent experiments, shown as the mean change percentage relative to Adwt control group ± S.D. * P<0.05 compared with the Adwt group, Student's t-test.