Figure 4. Effects of viral replication on pRb and CDK inhibitors.

A549 cells were mock-infected or infected with AdGFP, Adwt, or Adhz63 and collected at 24 h or 48 h p.i., followed by Western blot analysis. Cell lysates were immunoblotted for (A) pRb, phospho-pRb (p-pRb) at S612, T821 and S795 or (B) p21 and p27. Actin was used as a loading control. * P<0.05 compared with the mock-control group, Student's t-test.