Figure 8. Effects of CDK2-specific siRNA on Ad replication in WI-38 human lung fibroblast cells.

WI-38 cells were transfected with CDK2 siRNA duplex or a duplex of non-specific control siRNA. At 48 h after transfection cells were infected with AdGFP, Adwt, or Adhz63 at an MOI of 5. (A) CPE was photographed at 72 h p.i. All microscopy was originally at a magnification of x100. (B) The viral titers were determined at 72 h p.i. with the infection unit method. The values are means ± S.D. of independent triplicate. * P<0.05 compared with the control group, Student's t-test. (C) Cells were collected at 48 h p.i. and viral DNA synthesis was determined by Southern blot. Cell lysates were immunoblotted (D) for adenovirus type 5 capsid proteins and for (E) CDK2, pCDK2 T160, pRb, p-pRb S612 and cyclin EL. Actin was used as a loading control.